Re-expression of ABP-120 rescues cytoskeletal, motility, and phagocytosis defects of ABP-120- Dictyostelium mutants.
نویسندگان
چکیده
منابع مشابه
Genetic deletion of ABP-120 alters the three-dimensional organization of actin filaments in Dictyostelium pseudopods
This study extends the observations on the defects in pseudopod formation of ABP-120+ and ABP-120- cells by a detailed morphological and biochemical analysis of the actin based cytoskeleton. Both ABP-120+ and ABP-120- cells polymerize the same amount of F-actin in response to stimulation with cAMP. However, unlike ABP-120+ cells, ABP-120- cells do not incorporate actin into the Triton X-100-ins...
متن کاملTargeted disruption of the ABP-120 gene leads to cells with altered motility
The actin-binding protein ABP-120 has been proposed to play a role in cross-linking F-actin filaments during pseudopod formation in motile Dictyostelium amebas. We have tested this hypothesis by analyzing the phenotype of mutant cell lines which do not produce ABP-120. Two different transformation vectors capable of targeted disruption of the ABP-120 gene locus have been constructed using a por...
متن کاملPhotosensory and thermosensory responses in Dictyostelium slugs are specifically impaired by absence of the F-actin cross-linking gelation factor (ABP-120)
Chemotactic aggregation of starving amoebae of Dictyostelium discoideum leads to formation of a motile, multicellular organism - the slug - whose anterior tip controls its phototactic and thermotactic behaviour. To determine whether proteins that regulate the in vitro assembly of actin are involved in these responses, we tested phototaxis and thermotaxis in mutant slugs in which the gene encodi...
متن کاملDictyostelium discoideum mutants with conditional defects in phagocytosis
We have isolated and characterized Dictyostelium discoideum mutants with conditional defects in phagocytosis. Under suspension conditions, the mutants exhibited dramatic reductions in the uptake of bacteria and polystyrene latex beads. The initial binding of these ligands was unaffected, however, indicating that the defect was not in a plasma membrane receptor: Because of the phagocytosis defec...
متن کاملAnatomic Variability of 120 L5 Spondylolytic Defects
Study Design Adult human osteologic specimens were assessed for spondylolytic defects and characterized. Objectives To characterize and determine the prevalence of spondylolytic defects in an osteological collection. Methods Lumbar vertebrae from the Hamann-Todd Osteological Collection at the Cleveland Museum of Natural History were examined. Digital images of specimens with L5 isthmic spondylo...
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ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 1996
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.7.5.803